TNES-Puffer
Gesprochen "Tünnes". 0,1N NaOH / 0,5% SDS in [Puffer TE.html]TE-Puffer]. Verwendet in Mini-Prep (Plasmid-DNA-Isolation aus E. coli).
| 5 ml | 10 ml | 15 ml | |
|---|---|---|---|
| 1N NaOH | 500 µl | 1 ml | 1,5 ml |
| 20% SDS | 125 µl | 250 µl | 375 µl |
| 1× TE-Puffer | 4,38 ml | 8,8 ml | 13 ml |